Voltammetric assessment of dopamine clearance in the absence of the dopamine transporter: no contribution of other transporters in core or shell of nucleus accumbens
by
Mateo Y, Budygin EA, John CE, Banks ML, Jones SR.
Department of Physiology and Pharmacology,
Wake Forest University School of Medicine,
Medical Center Blvd.,
Winston-Salem, NC 27157, USA.
J Neurosci Methods. 2004 Dec 30;140(1-2):183-

ABSTRACT

Cocaine elevates dopamine (DA) in the nucleus accumbens (NAc) by blocking the uptake of DA through the DA transporter (DAT). It is commonly believed that the reinforcing properties of cocaine depend upon interaction with the DAT, however, cocaine is still reinforcing in mice with a genetic deletion of the DAT (DAT-KO mice). Although cocaine continues being able to elevate DA in the NAc of these mice, this mechanism is unclear. The present voltammetric study in brain slices was designed to examine the role of the norepinephrine and serotonin transporters in removing DA from the extracellular space in the NAc of DAT-KO mice. We found no effects of any monoamine uptake inhibitors, including cocaine (10muM), desipramine (10muM) or fluoxetine (10muM) on the clearance of DA in these mice. Therefore, it appears that there is no compensatory uptake of DA by alternative transporters either in core or shell of the nucleus accumbens of DAT-KO mice.

BP 897
Dopamine
GBR 12909
Oral cocaine
Coke v crack
Adrenergic crisis
The pleasure centres
The nucleus accumbens
Dopamine/D1receptors
Dopamine/D2 receptors
Dopamine/D3 receptors
Dopamine/D4 receptors
Dopamine/D5 receptors
Dopamine efflux and abused drugs

01 02 03 04 05 06 07 08 09 10 11 12
13 14 15 16 17 18 19 20 21 22 23 24

Refs
HedWeb
Future Opioids
BLTC Research
Utopian Surgery?
Paradise-Engineering
The Hedonistic Imperative
MDMA: Utopian Pharmacology
When Is It Best to Take Crack Cocaine?

The Good Drug Guide
The Responsible Parent's Guide To
Healthy Mood Boosters For All The Family